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Valiant Co Ltd schwann cell media
Rat <t>Schwann</t> <t>cell</t> migration into hydrogels with magnetically templated channels is influenced by channel diameter. Migrating rat Schwann cells seeded in hydrogels templated with non-aligned (A, D) 32 µm diameter MAMs, (B, E) 62 µm diameter MAMs, and (C, F) 90 µm MAMs and imaged at (A–C) day 1 and (D–F) day 3 after seeding. Migrating rat Schwann cells seeded in hydrogels templated with aligned (G, L) 32 µm diameter MAMs, (H, K) 62 µm diameter MAMs, and (I, L) 90 µm diameter MAMs and imaged at (G–I) day 1 and (J–L) day 3 after seeding. Cellular distributions in templated hydrogels at day 1 and day 3 for hydrogel templated with (M) 32, (N) 62, and (O) 90 µm MAMs. Cell count data are plotted as the minimum observation, lower 25% quartile (Q1), median, mean, upper 75% quartile (Q3), and maximum observation ( n = 3 per group). All ticks represent 100 µm intervals.
Schwann Cell Media, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Tuning hydrogel properties and Schwann cell behavior through microchannel size control in magnetically templated hydrogels"

Article Title: Tuning hydrogel properties and Schwann cell behavior through microchannel size control in magnetically templated hydrogels

Journal: Biomaterials Science

doi: 10.1039/d5bm01573a

Rat Schwann cell migration into hydrogels with magnetically templated channels is influenced by channel diameter. Migrating rat Schwann cells seeded in hydrogels templated with non-aligned (A, D) 32 µm diameter MAMs, (B, E) 62 µm diameter MAMs, and (C, F) 90 µm MAMs and imaged at (A–C) day 1 and (D–F) day 3 after seeding. Migrating rat Schwann cells seeded in hydrogels templated with aligned (G, L) 32 µm diameter MAMs, (H, K) 62 µm diameter MAMs, and (I, L) 90 µm diameter MAMs and imaged at (G–I) day 1 and (J–L) day 3 after seeding. Cellular distributions in templated hydrogels at day 1 and day 3 for hydrogel templated with (M) 32, (N) 62, and (O) 90 µm MAMs. Cell count data are plotted as the minimum observation, lower 25% quartile (Q1), median, mean, upper 75% quartile (Q3), and maximum observation ( n = 3 per group). All ticks represent 100 µm intervals.
Figure Legend Snippet: Rat Schwann cell migration into hydrogels with magnetically templated channels is influenced by channel diameter. Migrating rat Schwann cells seeded in hydrogels templated with non-aligned (A, D) 32 µm diameter MAMs, (B, E) 62 µm diameter MAMs, and (C, F) 90 µm MAMs and imaged at (A–C) day 1 and (D–F) day 3 after seeding. Migrating rat Schwann cells seeded in hydrogels templated with aligned (G, L) 32 µm diameter MAMs, (H, K) 62 µm diameter MAMs, and (I, L) 90 µm diameter MAMs and imaged at (G–I) day 1 and (J–L) day 3 after seeding. Cellular distributions in templated hydrogels at day 1 and day 3 for hydrogel templated with (M) 32, (N) 62, and (O) 90 µm MAMs. Cell count data are plotted as the minimum observation, lower 25% quartile (Q1), median, mean, upper 75% quartile (Q3), and maximum observation ( n = 3 per group). All ticks represent 100 µm intervals.

Techniques Used: Migration, Cell Characterization

Rat Schwann cell morphology in magnetically templated hydrogels is influenced by channel diameter. Confocal fluorescent images of Schwann cells in hydrogels templated with (A, J) 32, (B, K) 62, and (C, L) 90 µm MAMs at day 1 (A–C) and day 3 (J–L). Top view of reconstructed surfaces using IMARIS in (D) 32, (E) 62, and (F) 90 µm MAM templated hydrogels at day 1 and day 3 (M–O). Side view of reconstructed surfaces using IMARIS in (G) 32, (H) 62, and (I) 90 µm MAM templated hydrogels at day 1 and day 3 (P–R). White bars at the top represent the respective MAM diameter size during templating for comparison of the cell structure.
Figure Legend Snippet: Rat Schwann cell morphology in magnetically templated hydrogels is influenced by channel diameter. Confocal fluorescent images of Schwann cells in hydrogels templated with (A, J) 32, (B, K) 62, and (C, L) 90 µm MAMs at day 1 (A–C) and day 3 (J–L). Top view of reconstructed surfaces using IMARIS in (D) 32, (E) 62, and (F) 90 µm MAM templated hydrogels at day 1 and day 3 (M–O). Side view of reconstructed surfaces using IMARIS in (G) 32, (H) 62, and (I) 90 µm MAM templated hydrogels at day 1 and day 3 (P–R). White bars at the top represent the respective MAM diameter size during templating for comparison of the cell structure.

Techniques Used: Comparison

Related Articles

In Vitro:

Article Title: Tuning hydrogel properties and Schwann cell behavior through microchannel size control in magnetically templated hydrogels
Article Snippet: Channel length distributions, channel volume density, and average channel diameter were quantified as a function of MAM size using IMARIS. .. For in vitro culture, hydrogels underwent a sterile 1× PBS equilibration for 1 d, followed by equilibration in complete Schwann cell media for 1 d (10% fetal bovine serum, 1% penicillin–streptomycin–amphotericin B [MP Biomedicals, 091674049], 20 μg mL −1 bovine pituitary extract, 4 μM forskolin, and 10 ng mL −1 fibroblast growth factor in Dulbecco's Modified Eagle's medium). ..

Sterility:

Article Title: Tuning hydrogel properties and Schwann cell behavior through microchannel size control in magnetically templated hydrogels
Article Snippet: Channel length distributions, channel volume density, and average channel diameter were quantified as a function of MAM size using IMARIS. .. For in vitro culture, hydrogels underwent a sterile 1× PBS equilibration for 1 d, followed by equilibration in complete Schwann cell media for 1 d (10% fetal bovine serum, 1% penicillin–streptomycin–amphotericin B [MP Biomedicals, 091674049], 20 μg mL −1 bovine pituitary extract, 4 μM forskolin, and 10 ng mL −1 fibroblast growth factor in Dulbecco's Modified Eagle's medium). ..

Modification:

Article Title: Tuning hydrogel properties and Schwann cell behavior through microchannel size control in magnetically templated hydrogels
Article Snippet: Channel length distributions, channel volume density, and average channel diameter were quantified as a function of MAM size using IMARIS. .. For in vitro culture, hydrogels underwent a sterile 1× PBS equilibration for 1 d, followed by equilibration in complete Schwann cell media for 1 d (10% fetal bovine serum, 1% penicillin–streptomycin–amphotericin B [MP Biomedicals, 091674049], 20 μg mL −1 bovine pituitary extract, 4 μM forskolin, and 10 ng mL −1 fibroblast growth factor in Dulbecco's Modified Eagle's medium). ..



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Rat <t>Schwann</t> <t>cell</t> migration into hydrogels with magnetically templated channels is influenced by channel diameter. Migrating rat Schwann cells seeded in hydrogels templated with non-aligned (A, D) 32 µm diameter MAMs, (B, E) 62 µm diameter MAMs, and (C, F) 90 µm MAMs and imaged at (A–C) day 1 and (D–F) day 3 after seeding. Migrating rat Schwann cells seeded in hydrogels templated with aligned (G, L) 32 µm diameter MAMs, (H, K) 62 µm diameter MAMs, and (I, L) 90 µm diameter MAMs and imaged at (G–I) day 1 and (J–L) day 3 after seeding. Cellular distributions in templated hydrogels at day 1 and day 3 for hydrogel templated with (M) 32, (N) 62, and (O) 90 µm MAMs. Cell count data are plotted as the minimum observation, lower 25% quartile (Q1), median, mean, upper 75% quartile (Q3), and maximum observation ( n = 3 per group). All ticks represent 100 µm intervals.
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Image Search Results


Rat Schwann cell migration into hydrogels with magnetically templated channels is influenced by channel diameter. Migrating rat Schwann cells seeded in hydrogels templated with non-aligned (A, D) 32 µm diameter MAMs, (B, E) 62 µm diameter MAMs, and (C, F) 90 µm MAMs and imaged at (A–C) day 1 and (D–F) day 3 after seeding. Migrating rat Schwann cells seeded in hydrogels templated with aligned (G, L) 32 µm diameter MAMs, (H, K) 62 µm diameter MAMs, and (I, L) 90 µm diameter MAMs and imaged at (G–I) day 1 and (J–L) day 3 after seeding. Cellular distributions in templated hydrogels at day 1 and day 3 for hydrogel templated with (M) 32, (N) 62, and (O) 90 µm MAMs. Cell count data are plotted as the minimum observation, lower 25% quartile (Q1), median, mean, upper 75% quartile (Q3), and maximum observation ( n = 3 per group). All ticks represent 100 µm intervals.

Journal: Biomaterials Science

Article Title: Tuning hydrogel properties and Schwann cell behavior through microchannel size control in magnetically templated hydrogels

doi: 10.1039/d5bm01573a

Figure Lengend Snippet: Rat Schwann cell migration into hydrogels with magnetically templated channels is influenced by channel diameter. Migrating rat Schwann cells seeded in hydrogels templated with non-aligned (A, D) 32 µm diameter MAMs, (B, E) 62 µm diameter MAMs, and (C, F) 90 µm MAMs and imaged at (A–C) day 1 and (D–F) day 3 after seeding. Migrating rat Schwann cells seeded in hydrogels templated with aligned (G, L) 32 µm diameter MAMs, (H, K) 62 µm diameter MAMs, and (I, L) 90 µm diameter MAMs and imaged at (G–I) day 1 and (J–L) day 3 after seeding. Cellular distributions in templated hydrogels at day 1 and day 3 for hydrogel templated with (M) 32, (N) 62, and (O) 90 µm MAMs. Cell count data are plotted as the minimum observation, lower 25% quartile (Q1), median, mean, upper 75% quartile (Q3), and maximum observation ( n = 3 per group). All ticks represent 100 µm intervals.

Article Snippet: For in vitro culture, hydrogels underwent a sterile 1× PBS equilibration for 1 d, followed by equilibration in complete Schwann cell media for 1 d (10% fetal bovine serum, 1% penicillin–streptomycin–amphotericin B [MP Biomedicals, 091674049], 20 μg mL −1 bovine pituitary extract, 4 μM forskolin, and 10 ng mL −1 fibroblast growth factor in Dulbecco's Modified Eagle's medium).

Techniques: Migration, Cell Characterization

Rat Schwann cell morphology in magnetically templated hydrogels is influenced by channel diameter. Confocal fluorescent images of Schwann cells in hydrogels templated with (A, J) 32, (B, K) 62, and (C, L) 90 µm MAMs at day 1 (A–C) and day 3 (J–L). Top view of reconstructed surfaces using IMARIS in (D) 32, (E) 62, and (F) 90 µm MAM templated hydrogels at day 1 and day 3 (M–O). Side view of reconstructed surfaces using IMARIS in (G) 32, (H) 62, and (I) 90 µm MAM templated hydrogels at day 1 and day 3 (P–R). White bars at the top represent the respective MAM diameter size during templating for comparison of the cell structure.

Journal: Biomaterials Science

Article Title: Tuning hydrogel properties and Schwann cell behavior through microchannel size control in magnetically templated hydrogels

doi: 10.1039/d5bm01573a

Figure Lengend Snippet: Rat Schwann cell morphology in magnetically templated hydrogels is influenced by channel diameter. Confocal fluorescent images of Schwann cells in hydrogels templated with (A, J) 32, (B, K) 62, and (C, L) 90 µm MAMs at day 1 (A–C) and day 3 (J–L). Top view of reconstructed surfaces using IMARIS in (D) 32, (E) 62, and (F) 90 µm MAM templated hydrogels at day 1 and day 3 (M–O). Side view of reconstructed surfaces using IMARIS in (G) 32, (H) 62, and (I) 90 µm MAM templated hydrogels at day 1 and day 3 (P–R). White bars at the top represent the respective MAM diameter size during templating for comparison of the cell structure.

Article Snippet: For in vitro culture, hydrogels underwent a sterile 1× PBS equilibration for 1 d, followed by equilibration in complete Schwann cell media for 1 d (10% fetal bovine serum, 1% penicillin–streptomycin–amphotericin B [MP Biomedicals, 091674049], 20 μg mL −1 bovine pituitary extract, 4 μM forskolin, and 10 ng mL −1 fibroblast growth factor in Dulbecco's Modified Eagle's medium).

Techniques: Comparison